Tirupati Railway Station is connected by trains from Bangalore, Chennai, Visakhapatnam, Madurai and Mumbai. A major railhead nearby is Renigunta, which is 15 km away. Renigunta is located in Trivandrum – Delhi route, which is connected to all cities in India by rail.
| Starting Station | Train | Arrival at Tirupati | Service Days |
| Bangalore (SBC) 5 more trains | Seshadri Expres (7209) | 07:55 PM (duration: 6h 35m) | All Days |
| Chennai (MAS) 1 more train | Sapthagiri Exp (6057) | 09:30 AM (duration: 3h 5m) | All Days |
| Tirupathi Exp (6053) | 05:00 PM (duration: 3h 10m) | All Days | |
| Delhi (NDLS) 5 more trains | Kerala Express (2626) | 09:05 PM (duration: 33h 35m) | All Days |
| Hyderabad (SC) 6 more trains | Narayanadri Exp (2734) | 06:05 AM (duration: 12h) | All Days |
| Padmavati Exp (2764) | 07:00 AM (duration: 12h 30m) | Tue, Thu, Fri, Sat & Sun | |
| Mumbai (CSTM) | Nagarcoil Exp (6351) | 10:45 AM (duration: 22h 40m) | Tue & Sat |
| Kanyakumari Exp (6381) | 02:30 PM (duration: 22h 45m) | All Days | |
| Adilabad (ADB) | Krishna Exp (7406) | 09:25 PM (duration: 24h 25m) | All Days |
| Bhubaneswar (BBS) 3 more trains | Bbs Ypr Sup Exp (2845) | 03:45 AM (duration: 20h 15m) | Sun |
| Bilaspur (BSP) | Bsp Tpty Expres (7481) | 04:05 PM (duration: 30h) | Tue & Sat |
| Chamarajanagar (CMNR) | Tirupati Passr (213) | 05:35 AM (duration: 14h 35m) | All Days |
| Chittoor (CTO) 15 more trains | Venkatadri Exp (2798) | 06:30 PM (duration: 1h) | All Days |
| Coimbatore (CBE) 9 more trains | Cbe Tirupaty Ex (0623) | 07:30 AM (duration: 8h 55m) | Tue |
| Ernakulam (ERS) 6 more trains | Nizamuddin Exp (2645) | 07:30 AM (duration: 12h 40m) | Sat |
| Ers Patna Exp (6359) | 12:10 PM (duration: 13h 25m) | Sat | |
| Guntur (GNT) 2 more trains | Gnt Tpty Pas (440) | 11:45 AM (duration: 10h 45m) | All Days |
| H Nizamuddin (NZM) 3 more trains | Ers Millenum Ex (2646) | 05:10 PM (duration: 35h 10m) | Tue |
| Swarna Jayanti (2644) | 05:10 PM (duration: 35h 10m) | Fri | |
| Hatia (HTE) | Hte Ypr Express (2835) | 10:00 PM (duration: 27h 40m) | Tue & Sun |
| Howrah (HWH) | Hwh Pdy Exp (2867) | 01:20 AM (duration: 25h 50m) | Sun |
| Hwh Ypr Express (2863) | 11:40 PM (duration: 27h 5m) | All Days | |
| Hubli (UBL) 1 more train | Ubl Tirupati Pa (271) | 09:00 PM (duration: 15h) | All Days |
| Hyderabad Decan (HYB) | Sabari Exp (7230) | 12:30 AM (duration: 12h 30m) | All Days |
| Rayalaseema Exp (7429) | 08:45 AM (duration: 15h 20m) | All Days | |
| Jammu (JAT) | Navyug Express (6688) | 01:20 AM (duration: 49h 35m) | Thu |
| Himsagar Exp (6318) | 01:20 AM (duration: 49h 35m) | Mon | |
| Kacheguda (KCG) 2 more trains | Venkatadri Exp (2797) | 07:30 AM (duration: 11h 25m) | All Days |
| Kakinada (CCT) 1 more train | Sheshadri Exp (7210) | 05:25 AM (duration: 11h 50m) | All Days |
| Kakinada Port (COA) | Tirupati Psgr (274) | 02:40 AM (duration: 19h 40m) | All Days |
| Kanyakumari (CAPE) | Cape Mumbai Exp (6382) | 03:20 AM (duration: 21h 50m) | All Days |
| Himsagar Exp (6317) | 09:08 AM (duration: 19h 8m) | Fri | |
| Kolhapur (KOP) | Haripriya Exp (7416) | 08:45 AM (duration: 21h 10m) | All Days |
| Machelipatnam (MTM) | Mtm Tpty Exp (7402) | 04:35 AM (duration: 9h 35m) | All Days |
| Mangalore Cntl (MAQ) | Navyug Exp (6687) | 09:08 AM (duration: 16h 48m) | Mon |
| Nagercoil (NCJ) 2 more trains | Gurudev Express (2659) | 09:08 AM (duration: 18h 33m) | Sun |
| Ncj Mumbai Exp (6352) | 09:40 PM (duration: 16h 50m) | Thu & Sun | |
| Narasapuram (NS) | Ns Tpty Exp (7402A) | 04:35 AM (duration: 11h 20m) | All Days |
| Okha (OKHA) | Rameswaram Exp (6734) | 06:10 AM (duration: 48h 5m) | Tue |
| Patna (PNBE) | Pnbe Ers Expres (6360) | 09:50 AM (duration: 41h 20m) | Tue |
| Pondicherry (PDY) | Pdy Howrah Exp (2868) | 07:30 PM (duration: 8h) | Wed |
| Puri (PURI) 4 more trains | Puri Tpty Exp (7479) | 04:05 PM (duration: 27h 5m) | Mon, Wed, Thu, Fri & Sun |
| Rameswaram (RMM) 3 more trains | Rmm Okha Expres (6733) | 11:10 AM (duration: 14h 20m) | Fri |
| Shalimar (SHM) | Gurudev Express (2660) | 03:45 AM (duration: 28h 45m) | Wed |
| Tatanagar Jn (TATA) | Tata Ypr Exp (2889) | 10:00 PM (duration: 27h 25m) | Fri |
| Tirunelveli (TEN) 1 more train | Ten Jammu Exp (06317) | 09:08 AM (duration: 17h 23m) | Mon & Fri |
| Ten Jammu Exp (06687) | 09:08 AM (duration: 17h 23m) | Mon | |
| Trivandrum (TVC) 4 more trains | Sabari Express (7229) | 12:30 AM (duration: 17h 15m) | All Days |
| Kerala Express (2625) | 03:50 AM (duration: 16h 35m) | All Days | |
| Visakhapatnam (VSKP) 6 more trains | Tirumala Exp (7488) | 05:00 AM (duration: 15h 10m) | All Days |
| Yesvantpur Jn (YPR) 2 more trains | Ypr Howrah Exp (2864) | 02:30 AM (duration: 6h 55m) | All Days |
| Ypr Tata Exp (2890) | 02:35 PM (duration: 6h 5m) | Mon |
